Interprofessional Journal of Health Sciences. Vol.11 No.1 , January - June 2013.

COMPARATIVE EVALUATION OF RP-HPLC AND TLC-DENSITOMETRIC METHOD FOR DETERMINATION OF BARAKOL CONTENT IN SENNA SIAMEA LEAVES

Apirak Sakunpak , Chaowalit Monton, Pathamaporn Pathompak, Sukanya Settharaksa, Fameera Madaka, Siriporn Kittiwisut, and Krisana Krisintu

Abstract

Using RP-HPLC and TLC-densitometric for quantitative determination of barakol in Senna siamea leaves was validated and compared. The HPLC method utilized a ZORBAX Eclipse Plus C18 column (3.5 µm, 100 mm × 4.6 mm i.d.) with a mixture of methanol and water (68:32 v/v) as the mobile phase at a flow rate of 1 ml/min, and UV detection at 245 nm. TLC-densitometric method was performed on precoated TLC plates, coated with silica gel 60F-254, with the CAMAG TLC scanner controlled by CAT software. A mixture of ethyl acetate, hexane and chloroform (40:40:20 v/v/v) were used as mobile phases and were also detected with UV at 372 nm. The parameters of linearity, intra-day and inter-day precision, accuracy, specificity, sensitivity LOD and LOQ of both methods were evaluated. Both assays provided good linearity, accuracy, reproducibility, and selectivity for determination of barakol. The quantitative results of both analytical methods did not show any statistically significant differences between them.

Keywords: RP-HPLC, TLC-densitometric method, Senna siamea; barakol

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