IJHS
Interprofessional Journal of Health Sciences
formerly Bulletin of Health, Science and Technology (BHST)
https://ijhs.rsu.ac.th
ISSN (Print) 2672-9628
ISSN (Online) 2672-9423
Interprofessional Journal of Health Sciences. Vol.11 No.2 , July - December 2013.
EFFECTS OF GAMMA IRRADIATION ON FREE RADICAL INDUCTION, MAJOR COMPONENTS AND TOXICITY OF ETHANOLIC EXTRACT FROM ANDROGRAPHIS PANICULATA (BURM. F.) NEES LEAVES
Piyanuch Thongphasuk and Jarunee Thongphasuk
Abstract
Gamma irradiation is an effective process to reduce the presence of microorganisms and insects, however, it might affect the chemical components and may hence alter biological activities of irradiated medicinal herbs. The objective of this research is to study the effect of gamma irradiation (10 and 25 kGy) on Andrographis paniculata (Burm. f.) Nees. Electron paramagnetic resonance spectroscopy (EPR) and high performance liquid chromatography (HPLC) were used to study the induced free radicals and andrographolide content, respectively. Total phenolic content and antioxidant activity were investigated by Folin-Ciocalteu method and 1,1- diphenyl-2-picryhydrazyl radical (DPPH) scavenging assay, respectively. Toxic effect was detected with Toxi-chromotest. The results showed that irradiation at the doses of 10 and 25 kGy significantly (P < 0.05) induced free radicals in the irradiated A. paniculata (Burm. f.) Nees. However, nonsignificant changes (P > 0.05) in andrographolide content, total phenolic content, antioxidant activity and toxicity were observed.
Keywords: gamma irradiation, Andrographis paniculata (Burm. f.) Nees, free radical