Interprofessional Journal of Health Sciences. Vol.14 No.1 , January - June 2016.

CYTOTOXIC, ANTIPROLIFERTIVE AND APOPTOTIC ACTIVITY OF SECONDARY METABOLITES FROM ASPERGILLUS UNQUIS CRI282-03 ON HUMAN COLON CANCER CELL LINE (COLO 205)
 

Nalinee Pradubyat, Suchada Jongrungruangchok, Thanapat Songsak, and Supakit Wongwiwatthananukit

Abstract

The purpose of this study is to study the antiproliferative and cytotoxic effect of  three secondary metabolites which are nidulin, aspergillusidone D, and unguinol from marine-derived fungi Aspergillus unguis CRI282-03 on colon cancer cells (COLO 205). In the course of a microbial screening process, A. unguis CRI282-03 was isolated from soil samples collected at the Royal Navy Base in Tub-La-Mu bay, Pang-nga Province, Thailand. The isolated fungus of interest was cultivated in Chulabhorn Research Institute (CRI). Nidulin, aspergillusidone D and unguinol were tested on COLO 205 cell. In addition, the cytotoxic activity of those three pure constituents were determined using MTT assay, the antiprolifertive as well as the anti-apoptotic activity were evaluated by flow cytometry method. The result from MTT assay showed that IC₅₀ value of nidulin, aspergillusidone D, and unguinol on COLO 205 cells were 15, 21 and 33 µM, respectively. Furthermore, nidulin provided anticancer activity through inducing apoptosis to COLO 205. From the performed assay nidulin showed greater antiproliferation activity than aspergillusidone D.

Keywords: Aspergillus unguis CRI282-03, nidulin, aspergilusidone D

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