Interprofessional Journal of Health Sciences. Vol.14 No.2 , July - December 2016.

CYTOTOXIC ACTIVITY OF 4H–CHROMENES ON COLO 205 HUMAN COLON ADENOCARCINOMA CELL LINE

Suchada Jongrungruangchok, Nalinee Pradubyat, Thanapat Songsak, and Supakit Wongwiwatthananukit

Abstract

4H-chromenes are synthetic benzopyran derivatives which have various pharmacological activities. This study aimed to investigate and compare the cytotoxic activity of three selected 4H-chromenes (compound I, II, and III) against human colon adenocarcinoma cell line (COLO 205) using MTT assay. Cells were treated with increasing concentrations of compound I, II, and III from 20 to 200 µM and incubated for 24 hours. The results were presented as the percentage of viable cells compared to control (untreated cells). At the concentration from 20 to 200 µM, compound I, II and III showed a significant decline in % mean viability on COLO 205 cells from 85.63% to 2.01%, 89.18% to 1.31%, and 68.03 % to 7.42%, respectively. Compound I, II and III significantly exhibited cytotoxic activity against COLO 205 cells with IC₅₀ of  26, 30, and 150 μM, respectively.

Keywords: 4H-chromenes, cytotoxic activity, human colon adenocarcinoma cell line (COLO 205)

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