Interprofessional Journal of Health Sciences. Vol.14 No.2 , July - December 2016.

ESSENTIAL OIL CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF PIPER RIBESOIDES LEAVES ENDEMIC TO THAILAND

Supawan Bunrathep, Suchada Jonrunruangchok, and Nijsiri Ruangrungsi

Abstract

The essential oil obtained by hydrodistillation on Piper ribesoides dried leaves was analyzes by Gas Chromatography-Mass Spectrometry (GC-MS).  The major constituents were β-bisabolene (37.63%), followed by a-curcumene (17.58%), and b-caryophyllene (13.01%).  According to biological activities, P. ribesoides essential oil was screened for antioxidant activity by DPPH radical scavenging method.  The result showed 100 μg/ml of this essential oil exhibited the inhibitory activity of 36.30±1.68%.  This essential oil also exhibited antimicrobial activity against Candida albicans with the inhibition zones about 15 mm at the concentration of 10 mg/ml, followed by gram positive bacteria (Staphylococcus aureus, Bacillus subtilis, and Micrococcus luteus) with the inhibition zones of 7-9 mm at the concentration of 10 mg/ml, and against gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa) with the inhibition zones of 7-9 mm at the concentration of 100 mg/ml.  In addition, this oil was non-toxic to brine shrimp with the LC₅₀ value > 1,000 μg/ml.

Keywords: Piper ribesoides, essential oil component, biological activities

Download Full Paper.