IJHS
Interprofessional Journal of Health Sciences
formerly Bulletin of Health, Science and Technology (BHST)
https://ijhs.rsu.ac.th
ISSN (Print) 2672-9628
ISSN (Online) 2672-9423
Interprofessional Journal of Health Sciences. Vol.15 No.1 , January - June 2017.
PROTEIN KINASE INHIBITORY PROPERTIES OF EXTRACTS DERIVED FROM BOCCONIA FRUTESCENS AND GOMPHOCARPUS PHYSOCARPUS
Kūpa‘aikekaiao Thomas, Mengke Zhang, Justin Reinicke, Jimmy Parker, Donna Ohora, Marisa M. Wall, Thanapat Songsak, Supakit Wongwiwatthananukit, and Leng Chee Chang
Abstract
Bocconia frutescens is an invasive shrub originally introduced to Hawai’i as an ornamental plant. Gomphocarpus physocarpus is a shrub that is native to southern Africa, also introduced to Hawai’i. These two plants were selected for study due to the possibility of alkaloid contents and biological activities. The objective of this study was to examine the protein kinase inhibitory activity (PKI) of crude methanol extracts derived from Bocconia frutescens and Gomphocarpus physocarpus using hyphae formation inhibition assay against Streptomyces 85E at concentrations of 20 μg/disk. Extracts that showed protein kinase inhibitory activity at this concentration were tested at lower concentrations. Results found that all B. frutescens extracts showed PKI activity, whereas none of the G. physocarpus showed biological activity. In addition, the presence of chelerythrine and berberine alkaloids in B. frutescens extracts were assessed during this study using thin layer chromatography (TLC) and liquid chromatography-massspectrometry (LC-MS) analysis. The results showed that both alkaloids were present in all B. frutescens extracts.
Keywords: Protein kinase inhibition, hyphae formation inhibition assay, Bocconia frutescens