Interprofessional Journal of Health Sciences. Vol.16 No.1 , January - June 2018.

ESTIMATION OF CENTELLOIDS AND TOTAL PHENOLIC CONTENT OF CENTELLA ASIATICA (L.) URB. USING COMPUTER SOFTWARE AND THEIR CORRELATION WITH ANTIOXIDANT ACTIVITY

Chaowalit Monton, Sukanya Settharaksa, Chaiyaporn Songsangsirisak, Niratcha Chuenwiset, Jirapornchai Suksaeree, and Chitradee Luprasong

Abstract

Centella asiatica (L.) Urb. is a herbal plant with wound healing property and several biological activities. The aims of this work were to estimate the centelloids and total phenolic content of Centella asiatica (L.) Urb. using computer software and investigate the linear correlation between centelloids or total phenolic content and the antioxidant activity. The two-factor spherical composite experimental design was performed to estimate the content of centelloids and total phenolic compound. Extraction temperature and extraction time were varied five levels from 40 to 60 °C and 60 to 120 min, respectively. The increment of temperature and time provided a high content of centelloids. The higher content of total phenolic compound was achieved at the high temperature and medium extraction time. The estimation equation of both two groups of compound fitted with quadratic model. The estimation was reliable and stable. The optimal condition provided the highest centelloids content was 59 °C and 108.1 min. While 55.7 °C and 77.4 min gave the highest total phenolic content. The correlation study using statistical software between centelloids or total phenolic content and antioxidant activity was performed, centelloids content had a negative effect on antioxidant activity from 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. But, no correlation was found from ferric ion reducing antioxidant power (FRAP) assay. Total phenolic content did not correlate with antioxidant activity from DPPH radical scavenging assay as well as FRAP assay. These results are valuable for our future work as a standard condition for extraction of Centella asiatica (L.) Urb. to obtain the high content of active chemical constituent.

Keywords: centelloids content, total phenolic content, Centella

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