Interprofessional Journal of Health Sciences. Vol.19 No.1 , January - June 2021.

DEVELOPMENT OF STABILITY MONITORING TECHNIQUE FOR VITAMIN C TABLETS USING GRAYSCALE ANALYSIS

Sumalee Wannachaiyasit and Phakdee Sukpornsawan

Abstract

The objective of this research is to investigate the instability of vitamin C tablets by monitoring their color change using grayscale analysis. Vitamin C tablets were prepared by direct compression. The tablets composed of vitamin C as an active ingredient, talcum and Aerosil® as glidants, and Avicel PH® 102 as a diluent and disintegrant, while polyvinylpyrrolidone K30 was used as a dry binder. The instability of the tablets was accelerated by keeping the tablets without any packaging at 40±2°C, 75±5% RH. The tablet discoloration was observed and the color recorded as an RGB (Red Green Blue) image, and then transformed to grayscale value. The tablets were also determined for remaining ascorbic acid content using high performance liquid chromatography (HPLC). The results showed that when the storage time increased, the tablet color changed from white to brown, with a decrease in grayscale value, and a reduction in ascorbic acid content was observed. The results indicated that the change in grayscale value correlated with the instability of vitamin C in the tablets. This research showed that grayscale analysis could be a useful technique for monitoring the instability of vitamin C tablets.

Keywords: vitamin C tablets, tablet discoloration, grayscale analysis

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